Resistance and Sensitivity of Some Bacterial Strains Isolated from Hospital Wastewater and Nile Water Using Chlorination and Some Antibiotics in Cairo (Egypt)

A total of 394 bacterial isolates were isolated from Kasr AlAiny hospital wastewater effluent and river Nile water in Cairo (Egypt). Each 2 isolates from both sources were mixed in co-cultures and incubated (at room temperature) in sterilized Nile water for 48 hours under normal laboratory condition, taking into consideration the same gram staining and the different species. In addition, some isolates (before and after transfer of antibiotic resistance features tested) were exposed to chlorine dose (5ppm) for 120min at room temperature. The results showed that Staphylococcus aureus, Pseudomonas aeruginosa and Streptococcus faecalis converted from antibiotics sensitive to antibiotics resistant when mixed with Streptococcus faecalis, E. coli and Staphylococcus aureus, respectively for Naldixic Acid (30 μg), Gentamycin (10 μg), Ampicillin (30 μg) and Amoxicillin (30 μg). In addition, these bacteria became more survival when treated with chlorine. On the other hand, the results demonstrated that these bacteria was sensitive when exposed to Erythromycin (15 μg), Tetracycline (30 μg), Lincomycine (15 μg) and Rifamipicin (50 μg). Moreover, Salmonella typhi and E. coli isolated from Nile water were sensitive to chlorine (5 ppm) and antibiotics tested in both single and mixed cultures. Generally, the strains isolated from wastewater were more survival in chlorine dose tested than the bacterial isolates from Nile water samples. So, hospital effluent must be treated before delivering to Egyptian aquatic environment to protect public health. [Osman, G. A., Hassan, H. M. and Kamel, M. M. Resistance and Sensitivity of Some Bacterial Strains Isolated from Hospital Wastewater and Nile Water Using Chlorination and Some Antibiotics in Cairo (Egypt). Journal of American Science 2011;7(9):1033-1041]. (ISSN: 1545-1003). http://www.americanscience.org.